Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus.
Identifieur interne : 000038 ( Main/Exploration ); précédent : 000037; suivant : 000039Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus.
Auteurs : Olga Bondarenko ; Borys Dzyuba ; Marek Rodina ; Jacky CossonSource :
- Reproduction, fertility, and development [ 1031-3613 ] ; 2016.
Abstract
The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated 'TS after IVM' (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1-2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.
DOI: 10.1071/RD16145
PubMed: 27246727
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000064
- to stream PubMed, to step Curation: 000064
- to stream PubMed, to step Checkpoint: 000064
- to stream Ncbi, to step Merge: 000709
- to stream Ncbi, to step Curation: 000709
- to stream Ncbi, to step Checkpoint: 000709
- to stream Main, to step Merge: 000038
- to stream Main, to step Curation: 000038
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus.</title><author><name sortKey="Bondarenko, Olga" sort="Bondarenko, Olga" uniqKey="Bondarenko O" first="Olga" last="Bondarenko">Olga Bondarenko</name></author><author><name sortKey="Dzyuba, Borys" sort="Dzyuba, Borys" uniqKey="Dzyuba B" first="Borys" last="Dzyuba">Borys Dzyuba</name></author><author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name></author><author><name sortKey="Cosson, Jacky" sort="Cosson, Jacky" uniqKey="Cosson J" first="Jacky" last="Cosson">Jacky Cosson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27246727</idno><idno type="pmid">27246727</idno><idno type="doi">10.1071/RD16145</idno><idno type="wicri:Area/PubMed/Corpus">000064</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000064</idno><idno type="wicri:Area/PubMed/Curation">000064</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000064</idno><idno type="wicri:Area/PubMed/Checkpoint">000064</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000064</idno><idno type="wicri:Area/Ncbi/Merge">000709</idno><idno type="wicri:Area/Ncbi/Curation">000709</idno><idno type="wicri:Area/Ncbi/Checkpoint">000709</idno><idno type="wicri:doubleKey">1031-3613:2016:Bondarenko O:role:of:ca</idno><idno type="wicri:Area/Main/Merge">000038</idno><idno type="wicri:Area/Main/Curation">000038</idno><idno type="wicri:Area/Main/Exploration">000038</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus.</title><author><name sortKey="Bondarenko, Olga" sort="Bondarenko, Olga" uniqKey="Bondarenko O" first="Olga" last="Bondarenko">Olga Bondarenko</name></author><author><name sortKey="Dzyuba, Borys" sort="Dzyuba, Borys" uniqKey="Dzyuba B" first="Borys" last="Dzyuba">Borys Dzyuba</name></author><author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name></author><author><name sortKey="Cosson, Jacky" sort="Cosson, Jacky" uniqKey="Cosson J" first="Jacky" last="Cosson">Jacky Cosson</name></author></analytic><series><title level="j">Reproduction, fertility, and development</title><idno type="ISSN">1031-3613</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated 'TS after IVM' (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1-2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Bondarenko, Olga" sort="Bondarenko, Olga" uniqKey="Bondarenko O" first="Olga" last="Bondarenko">Olga Bondarenko</name><name sortKey="Cosson, Jacky" sort="Cosson, Jacky" uniqKey="Cosson J" first="Jacky" last="Cosson">Jacky Cosson</name><name sortKey="Dzyuba, Borys" sort="Dzyuba, Borys" uniqKey="Dzyuba B" first="Borys" last="Dzyuba">Borys Dzyuba</name><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000038 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000038 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:27246727
|texte= Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27246727" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |